This application lab is a sequential extension of the corresponding techniques lab (BME 3813 Cellular Engineering: Techniques). This course provides experience in advanced cellular engineering techniques for students who are already proficient in basic mammalian cell culture methods. Students, in consultation with the course instructor, will formulate and conduct independent project(s) to answer specific questions/hypothesis from selected research topics. Students will develop their own experimental plans, considering the experimental set up, timelines, number of replicates, experimental controls, and strategies to test their hypothesis. Students must use proper statistical method(s) to test their hypothesis and present their findings.